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Introduction to Different Microbial Bio-Insecticides and Their Application Rates
April 25, 2026 | by anisurrahaman235f@gmail.com
Iron-Chelating Compounds Produced by Microorganisms
April 25, 2026 | by anisurrahaman235f@gmail.com
Insoluble Phosphate-Solubilizing Microbial Fertilizers (PSM)
April 25, 2026 | by anisurrahaman235f@gmail.com
When to Use Azotobacter or Azospirillum
April 25, 2026 | by anisurrahaman235f@gmail.com
Nitrogen Fixation by Different Leguminous Crops in Soil
April 25, 2026 | by anisurrahaman235f@gmail.com
Effectiveness of Different Types of Beneficial Microbial Fertilizers
April 25, 2026 | by anisurrahaman235f@gmail.com
(c) In Enhancing the Effectiveness of Organic Manure:
April 25, 2026 | by anisurrahaman235f@gmail.com
| Technical Name | Nature of Organism | Trade / Commercial Names | Application Rate |
|---|---|---|---|
| Bacillus thuringiensis subsp. kurstaki (Btk) | Bacteria | Thuricide, Biobit, Hil-Bitk, Delfin, Dipel 8-L, Biolep, Halt, Bio-Amp, Fighter | Spray: 1 g/litre water |
| Beauveria bassiana 1.15% W.P. | Fungus | Biorin, Biosafe, Daman, Brevguard, Basina, Larvosel, Biopower, Dispel, Biolon, Biocatch, Mycotal, Ecosil | Spray: 1–2 g/litre water Soil application: 2.5 kg/ha + 100 kg organic manure |
| Verticillium lecanii 1.15% W.P. | Fungus | Biolon, Biocatch, Mycotal, Ecosil | Spray: 5 g/litre water |
| Metarhizium anisopliae 1.15% W.P. | Fungus | Biomet, Bio Magic, Metacin | Spray: 5 g/litre water Soil application: 1 kg/acre + 50 kg organic manure |
| Paecilomyces fumosoroseus 1.15% W.P. | Fungus | Priority, Bionematin | Spray: 5 g/litre water Soil application: 1.5 kg/acre + 50 kg neem cake |
| Paecilomyces lilacinus 1.15% W.P. | Fungus | Nemat, Bio-Nematon | Soil application: 3 kg/ha + 100 kg organic manure |
Virus-based Bio-insecticides
| Technical Name | Nature of Organism | Trade / Commercial Names | Application Rate |
|---|---|---|---|
| NPV (H) – Helicoverpa NPV (Virus) | Virus | Helicide, Heligard, Biovirus-H, Helivex, Helinash | 1 ml/litre water @ 100 LE/acre (Larval Equivalent) |
| NPV (S) – Spodoptera NPV (Virus) | Virus | Spodocide, Spodo-NPV, Biovirus-S, Spodovex, Spodonash | 1 ml/litre water @ 100 LE/acre |
| Granulosis Virus (GV) | Virus | Agribir, Modex, Biorakshak | 100 LE/acre @ 1 ml/litre water |
| Bacillus thuringiensis (Galleria) | Bacteria | Spectrin (for stored grain) | 0.5–1 g/sq. metre |
| Bacillus thuringiensis (israelensis) | Bacteria | Bactiside, Hil-Bti (for stored grain) | 0.5 g/metre or 1 g/litre water |
Bacteria and Fungi-based Bio-fungicides
| Technical Name | Nature of Organism | Trade / Commercial Names | Application Rate |
|---|---|---|---|
| Pseudomonas fluorescens 1.75% W.P. | Bacteria | Biomona, Pseudocel, Biocure-B, Bioshield, Pant Bio-Control Agent-2, Rakshak Nima-2, Sparsh | Seed treatment: 4–5 g/kg seed Soil application: 1–2 kg/acre + 100 kg organic manure Seedling dip: 20–25 g/litre water (25–30 minutes) |
| Bacillus subtilis | Bacteria | Biotek | Seed treatment: 4 g/kg seed Seedling dip: Similar to Pseudomonas |
| Trichoderma viride 1.15% W.P. | Fungus | Biocon, Kasana, Bioguard, Tricostar, Nisarg, Defence-SF, Fanginil, Tricoguard, Trico-X, Biocure-F | Seed treatment: 4–5 g/kg seed Soil treatment: 1–2 kg/acre + 100 kg organic manure Seedling dip: 20–25 g/litre water (25–30 minutes) |
| Trichoderma harzianum 1.15% W.P. | Fungus | Pant Bio-Control Agent-1, Niprot, Ecoderma, Supravit, Tricodex | Same as Trichoderma viride |
| Trichoderma viride + Trichoderma harzianum | Fungus | Biocure (B & F), Bioderma-F | Seed: 4–5 g/kg Soil: 1 kg/acre + 100 kg organic manure Seedling dip: 20–25 g/litre water (25–30 minutes) |
| Aspergillus niger (AN-27) | Fungus | Kalisena-SD, Kalisena-SL | Seed treatment: 10 g/kg seed |
Methods and Precautions for Application of Microbial Bio-insecticides, Bio-fungicides, and Bio-herbicides
The method of application of microbial products varies depending on the formulation, crop, and stage of the crop. However, the following general precautions and guidelines must be strictly followed:
- Always use the microbial product before its expiry date.
- During seed treatment, seedling treatment, or soil application, ensure that the microorganisms do not come into contact with chemical fertilizers or chemical pesticides/herbicides.
- Apply sufficient organic manure to the field before or along with microbial application.
- For Bt-based products, apply when the ambient temperature is below 30°C.
- Use only the specific NPV strain targeted against the particular pest; otherwise, it will be ineffective.
- Spray microbial insecticides and fungicides in the late afternoon or evening. Spraying in bright sunlight reduces effectiveness.
- The method of seed treatment, soil treatment, or seedling dip using microbial fungicides is similar to that used for biofertilizers.
- Do not apply any chemical sprays 5–7 days before or after applying microbial bio-pesticides.
Some Prominent Companies Producing and Marketing Microbial Bio-products in India
- Pest Control India, 23 Mirza Galib Street, Kolkata – 700 016
- Biotek International, Dutt House, 94 Chittaranjan Avenue, Kolkata – 700 012
- Krishi Rasayan, 234/3A, A.J.C. Bose Road, Kolkata – 700 020
- E.I.D. Parry, 4 Synagogue Street, Kolkata – 700 001
- Shri Sai Agri Bio Labs Pvt. Ltd., 4-48-10/1, Lawsons Bay Colony, Visakhapatnam – 530 017, Andhra Pradesh
- Total Agri-Care Concern Pvt. Ltd., 12/A, Netaji Subhash Road, Kolkata – 700 001
- Maxima Biotek Pvt. Ltd., 54 J.M.C. House, Ahmedabad – 380 006
- Elbitec Innovations Ltd. (M/s. Solace Croptek), Mylapore, Chennai
- Karnatak Agro Chemicals, Pune, Bengaluru – 560 058
- Excel Industries Limited, 706 Central Plaza, 2/6 Sarat Bose Road, Kolkata – 700 020
- T. Stanes & Co. Pvt. Ltd. (M/s. IndoPhil), Race Course Road, Chennai – 600 008
- Multiplex Biotek Pvt. Ltd., 2A Ganesh Chandra Avenue, Kolkata – 700 013
- Amit Biotek Pvt. Ltd., 21 Ballygunge Circular Road, Kolkata – 700 019
- R.P.C. Agro, 3 Portuguese Church Street, Kolkata – 700 001
- Super Agro Pvt. Ltd., 22B Satgachi Para, Kolkata – 700 002
- Shir Agro Bio-Fertilizer Creative Organization, Uttar Darua, Contai, Purba Medinipur
- Libis Agro Pvt. Ltd., 3A Dharmadas Road, Kalighat, Kolkata – 700 026
- Ajay Biotek Pvt. Ltd., 233A J.C. Road, Kolkata – 700 020
- P.J. Margo Pvt. Ltd., Crooked Lane, Kolkata – 700 069
- International Panacea Pvt. Ltd., 8/1 Middleton Row, Kolkata – 700 071
- Peak Chemical Industries Ltd., Sevoke Road, Siliguri – 734 001
Commercial Use of Beneficial Microorganisms in Agriculture
The commercial use of beneficial microorganisms in agriculture began several years ago. Many government, semi-government, and private organizations are now producing and marketing a wide range of microbial products. Farmers across the country are successfully using these products and achieving good results. Under the ‘Jaibo Gram’ (Organic Village) initiatives of the Government, farmers are also extensively using microbial fertilizers and bio-pesticides.
In the present context of agriculture, increasing the use of beneficial microorganisms has become essential to sustain farming. The richer the “buffer stock” of beneficial microbes in the soil, the more sustainable agriculture will become. This will increase productivity, reduce production costs, make farming more profitable, and keep the environment free from pollution.
Some Organizations Involved in Production and Sale of Microbial Fertilizers
- Pulse and Oilseed Research Centre, Ranibagan, Baharampur, Murshidabad – 742 102
- Nitrophix Laboratory, 25 Bansdroni Avenue, Kolkata – 700 070
- Biotek International Ltd., 94 Chittaranjan Avenue, Kolkata – 700 012
- Vivekananda Institute of Biotechnology, Nimpeeth Ashram, South 24 Parganas – 743 338
- Total Agri-Care Concern Pvt. Ltd., 12/A N.S. Road, Kolkata – 700 001
- Libis Agro Chem Pvt. Ltd., 3A Dharmadas Road, Kalighat, Kolkata – 700 026
- Nodule Research Laboratory, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia – 741 252
- Peak Chemical Industries Ltd., Sevoke Road, Siliguri – 734 001
- Krishak Bharati Cooperative Ltd., Block A, Lake Town, Kolkata – 700 089
- Shir Agro Bio-Fertilizer Creative Organization, Uttar Darua, Contai, Purba Medinipur
- Brahmaputra Valley Fertilizer Corporation Ltd., Milanpally, Siliguri – 734 405
- Shilavati Horticulture & Aquaculture, P-221/1 Strand Bank Road, Kolkata – 700 001
- Eastern Enterprises, 4/4 Poddar Nagar, Kolkata – 700 068
- Leela Agrotech, Rajibpur, North 24 Parganas – 743 702
- Multiplex Bio-Tech Pvt. Ltd., Gangotri Building, Ultadanga, Kolkata – 700 054
- Ansul Agro Chemical, Gangotri Building, Ultadanga, Kolkata – 700 054
- Nav Bharat Fertilizer Ltd., Gandhighar, Naxalbari, Darjeeling
- Micro Back India, O.G. Road, Shyamnagar, North 24 Parganas – 743 127
- IndoPhil Chemical Company, 34 Circus Avenue, Kolkata – 700 017
- Indian Farmers Fertilizer Cooperative (IFFCO), 8A J.C. Bose Road, Circular Court, Kolkata – 700 017